On-farm mycorrhizal inoculum
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USDA-ARS method (Douds et al.) circulated by the Rodale Institute to multiply native mycorrhizal fungi on the farm: a host grass grows for a season in bags of compost and vermiculite seeded with a little local soil, then roots and substrate become the inoculum for seedlings.
Application description: Mix the inoculum into seedling substrate or place it in the planting hole.
Dose: 1 volume of inoculum to 9 of substrate for cells of 50 cm³ or less; 1 to 19 for larger cells.
Phosphorus-rich compost must be diluted much more in vermiculite (up to 1:19 or 1:49). In the nursery, avoid fertilisers rich in soluble phosphorus, which hinder colonisation.
1. About 4 months before the last frost, sow bahiagrass in a nursery (soil and sand mix).
2. Collect soil from 4 or 5 spots in a natural area (hedgerow, woodlot) or a field without a host crop for 2 years, in the top 10 cm; sieve out stones and roots.
3. Mix compost and vermiculite, fill the bags and work in the 100 cm³ of soil.
4. Transplant 4 or 5 seedlings per bag.
5. Water and weed all season (5 to 10 minutes a week). Do not add soluble phosphorus.
6. At the end of the season, cut the dead leaves and let the bags overwinter.
7. To use, remove the root ball, shake the substrate into a tub, cut the roots with scissors and mix them in: this is the inoculum.
One growing season (about 4 months in the nursery, then summer).
Mix of compost, vermiculite and chopped roots, with fungal spores and filaments.
Rodale Institute, “How to inoculate arbuscular mycorrhizal fungi on the farm”, method by D. Douds (USDA-ARS): https://rodaleinstitute.org/science/articles/how-to-innoculate-arbuscular-mycorrhizal-fungi-on-the-farm-part-1/ — SARE, project LNE03-179: https://projects.sare.org/project-reports/lne03-179/ — Ingredient spotted on the Ecoagro technical sheet (Guamúchil, Sinaloa, Mexico). Recipe written in our own words.
Recipe written in our own words by biopreparados.es. Copyright and sources
Grégory Prybys
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Country: Argentina
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