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Mountain microorganisms

Mountain microorganisms

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MM, CMM — forest microorganisms, cultivation of mountain microorganisms

Inoculants Bio-input
commercialized
Fermentation Biological Most requested

Fermentation

Biological transformation of the plant material by microorganisms (yeasts, bacteria) in total or partial absence of oxygen, releasing and transforming active compounds over a longer period than the other techniques (days to weeks).

Process summary

1) Chop the plant material and submerge it in water, in a wide, non-airtight container (to let gases escape). 2) Stir daily during fermentation. 3) Let ferment from several days to several weeks, depending on ambient temperature, until it stops foaming. 4) Strain and dilute before use — the undiluted product can be very concentrated.

See all preparation methods

🟠 Not prohibited

A microbial inoculant that enhances soil biological activity, accelerates the decomposition of organic matter, and promotes the availability of nutrients for crops. It is also used as a compost activator and in biofertilizers.

Application

When to use: Routinely

Application description: Apply the liquid solution, diluted in water, by watering the soil or mixing it into compost; it can also be sprayed, in diluted form, onto the foliage. Avoid contact with direct sunlight and chlorinated water. Repeat the treatment every 30 days or at the start of each growing cycle.

Dose: Generally, 1 L of liquid per 10 to 20 L of water (1:10 to 1:20). Some sources recommend up to 1 part liquid to 4 parts water (20%) for the aeration-activated phase.

Tips

Discard any batch that shows signs of green, black, or pink mold: only white, cottony mold is usable. During the solid phase, seal the container tightly: oxygen can spoil the fermentation. Always use non-chlorinated water (chlorine inhibits microorganisms). The fermented liquid in a jug will keep for several months in a tightly sealed container in a cool place; the liquid activated by aeration should be used within the same day.

Preparation process

Collection: 1) Bury the cooked rice under the leaf litter in a native forest, in a spot where you can already see white mycelium in the soil. 2) Cover it with leaf litter and let it sit for 15 to 20 days. 3) The sample is ready when the rice is completely covered with a white, cottony mold; if mold of any other color appears, discard the sample.
Solid-state phase (optional): 4) Mix the forest litter or the culture with cornmeal, bran, or oats in a 20-liter airtight container. 5) Add the molasses dissolved in water (and, depending on the variation, honey production byproducts—honey foam or filtration residues—or sugar, 5% of the mixture’s weight), then check the moisture content using the fist test: the mixture should be moist without dripping. 6) Pack it down, seal it tightly, and let it ferment for 30 days in an airtight container. 7) To make an even larger batch, mix 10 kg of this product with the same proportions of fresh ingredients and let it ferment for another 30 days.
Liquid phase, as desired: 8a) In a container: dissolve the molasses (and, depending on the variation, honey foam, honey filtration residues, or sugar) in non-chlorinated water, add the bran, then the “capture” or solid phase, pour the mixture into an airtight container equipped with a pressure relief valve (siphon) and let it ferment for 15 to 30 days, until a sweet, fermented odor develops, similar to that of shisha or yeast. 8b) Aerated activation: dissolve 100 g of molasses in 20 L of unchlorinated water, wrap 500 g of the solid phase in a coarse cloth, submerge it in the solution, and aerate with an aquarium air pump for 24 to 72 hours.

Ingredients

  • Water ⓘ
  • Molasses ⓘ
  • Wheat or rice bran ⓘ
  • Native forest leaf litter and humus ⓘ
  • Cooked rice ⓘ
  • Corn flour ⓘ
  • Sucrose ⓘ
  • Oats or oat bran ⓘ
  • Honey production residues (skimmed foam, filtering residues) ⓘ
  • Step 1 – Collection: Fallen leaves and humus from a native forest, collected from under trees where abundant white mycelium (filamentous white mold) is present, and a handful of unsalted cooked rice as bait.
  • Step 2 – Solid phase (optional, for preservation and propagation): 3 kg of decomposing forest litter or the collected material, 6 kg of cornmeal, rice bran, or wheat bran (or, if unavailable, oats or oat bran), 1.5 kg of molasses, 2 L of unchlorinated water.
  • Step 3 – Liquid phase, as desired: in a container, 1 kg of capture or solid phase, 1 kg of rice bran or wheat bran (or oat bran, or cooked rice), 1 L of molasses and 20 L of unchlorinated water; or for aerated activation, 500 g of solid phase and 100 g of molasses in 20 L of unchlorinated water.
  • Variation on the standard recipe: Add honey production byproducts (honey foam, honey filtration residues) or sugar, amounting to 5% of the mixture’s weight, to both the solid and liquid phases.

Tools and equipment

  • Strainer or filter cloth
  • 20-litre airtight container
  • Aquarium air pump (aerator)
  • Airtight drum or fermenter with fermentation valve
  • Scale (kitchen or precision)
  • Container for collection in the forest (box or bucket with a lid). Airtight 20-liter container for the solid phase. Airtight container equipped with a pressure relief valve (hose and water bottle) for liquid fermentation. Coarse canvas cloth (30 x 30 cm) and an aquarium air pump for aerated activation. Scale. Strainer for filtering before use.

Preparation time

Capture: 15 to 20 days. Solid phase (optional): 30 to 60 days. Liquid phase: 15 to 30 days in a container, or 24 to 72 hours in an aerated activation process.

Product obtained

A liquid culture of beneficial native microorganisms (fungi and bacteria from forest soil), used in diluted form as an inoculant to activate biological activity in the soil and in fermented preparations.

Source / Reference

Standard agroecology methodology (Mountain Microorganisms / CMM), documented by institutions such as the CIA-UCR and INTA; dialnet.unirioja.es — “Propagation of Mountain Microorganisms” (protocol adapted for use in plant cultivation); López, Juanfran (2022). The Biofertiliser Manual, 2nd edition. www.juanfranlopez.com; SADER and INIFAP (2021). Manuales prácticos para la elaboración de bioinsumos, no. 13: Microorganismos de montaña (in Spanish). Technical Support Strategy, Mexican Ministry of Agriculture; Picado, J. and Añasco, A. Preparación y uso de abonos orgánicos sólidos y líquidos (in Spanish). Agricultura Orgánica series, CEDECO (Costa Rica); Prybys, Grégory. Field technical notes (in French); Garro Alfaro, J. E. (2016). El suelo y los abonos orgánicos (in Spanish). INTA Costa Rica, Ministry of Agriculture and Livestock; Chilean Ministry of the Environment, GEF Montaña project and INDAP (2021). Manual de bioinsumos para biofábrica (in Spanish).

Recipe written in our own words by biopreparados.es. Copyright and sources

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Contact person

Grégory Prybys
Tel: +33 7 75 76 37 70 — WhatsApp: +54 9 11 2157 0868
Country: Argentina

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Regulatory status

🟠 Not prohibited (made on the farm for own use)

No European text names “Mountain microorganisms”. Made on the farm for one’s own use, it falls under no authorisation regime: Regulation (EU) 2019/1009 and, in Spain, Royal Decree 506/2013 only cover products placed on the market. In organic farming, Regulation (EU) 2018/848 (Annex II, Part I, point 1.9.6) generally allows preparations of micro-organisms to improve soil condition or nutrient availability: the certification body will decide; there is no text specific to this preparation. Its ingredients appear on the organic input lists of several countries: see each country’s page.

Official texts

Official sources automatically checked on 20/10/2026.

What the icons mean: ✅ Authorised ⚠️ Tolerated 🟠 Not prohibited ⛔ Not authorised EU 🔎 Status being verified Full details →

Documentary information, checked on 20/10/2026. Not a substitute for legal advice; regulations may vary from country to country. See the regulations · Substances authorised in Europe

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